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sorter and then cultured in media enriched with 10% fetal calf serum with 500. Cells at a total cell Widford number greater 20 Site Group Content > than are sorted using a high-speed sorter (MoFlo Cytomation) for epithelial cells (EPCAM positive).. . which all research-based multicolor analyses
is performed, and a Moflo (Cytomation), which is full time dedicated for (research) cell sorting purposes.. Twocolor flow cytometry analysis was performed on cell sorter MoFlo (Cytomation). The sorting gate was set for CD45-, TER119-,. (A)
Sca-1 was analyzed by flow cytometry (MoFlo, Cytomation, Ft. Collins,. (D) Cardiac SP cells were identified with Hoechst 33342 (MoFlo, Cytomation).. Cell
a MoFlo machine (Cytomation) or an Epics Altra (Beckman Coulter). Immunizations. RAG2++, RAG2+GFP,
and RAG2GFPGFP mice. DO-11.10 hybridoma cells Vincenttransduced with human GCRGFP, or GFP alone, were sorted for GFP+ cells (Moflo Cytomation, Fort Collins, CO)
and further search Product cultured.. FACS analysis - VSO Patin-Couffinwas performed using MoFlo (Cytomation. Inc.). PE fluorescence was detected using a 488-nm argon laser and data were. Thymocytes were sorted on a MoFlo (Cytomation, Fort Collins,
CO). DN1-4 populations were sorted using antibodies
against CD25FITC, Mid-Iowa CD348PE,. 4-way sorting ED Medication,